吉林农业大学学报2013，Vol.35Issue(1)：40-45,6.
鲨烯环氧酶基因的克隆及其在人参根组织中的表达
Cloning of Encoding Squalene Epoxidase Gene and Its Expression in Panax ginseng Root Tissue
摘要
Abstract
Squalene epoxidase mRNA in Panax ginseng root tissue was cloned and its sequence analysis was conducted. Its expression level in ginseng root tissue of different years was detected with relative quantitative fluorescence Real - time PCR. The results showed that complete encoding sequence of SQE in ginseng root was 1 611 bp, which encoded 537 amino acid residues. Bioinformatic analysis of nu-cleotide and amino acid sequence based on SQE genes of squalene epoxidase on GenBank (No.: AB122078.1) showed that the homology was 99.75% and 99.81% respectively. Relative fluorescent quantitative PCR differential expression analysis showed that SQE gene had expression of varying intensities in root tissues of one-year, three-year, four-year and five-year ginseng. SQE genes manifested the highest expression level in root tissues of five-year ginseng.关键词
人参/鲨烯环氧酶基因/cDNA克隆/差异表达Key words
Panax ginseng/ squalene epoxidase gene/ cDNA cloning/ differential expression分类
生物科学引用本文复制引用
刘宁,田玉华,孟星宇,张连学,胡薇..鲨烯环氧酶基因的克隆及其在人参根组织中的表达[J].吉林农业大学学报,2013,35(1):40-45,6.基金项目
国家自然科学基金项目(30570185) （30570185）
