吉林农业大学学报2013，Vol.35Issue(1)：94-97,4.
产亚硝酸盐还原酶的乳酸菌的筛选及诱变
Screening and Mutagenesis of Lactic Acid Bacteria Producing Nitrite Reductase
摘要
Abstract
Nitrite content of food is one problem on food security, and it is always a hot issue to reduce nitrite levels in food. Forty-one strains of lactic acid bacteria were isolated from the northeast Korean-nationality pickled vegetables, and these bacteria can degrade nitrite. Then six strains which have better degradation ability were rescreened, and A2, B1, B10, Cc2 strains were determined to be Lactobacillus and No. D3, D5 strains to be Pediococcus Pearl. The nitrite degradation rate of B10 strain was 70.5% after 48 h at 37℃. In order to improve the nitrite degradation ability of B10 strain, 10-4dilution of the bacterial concentration was selected after UV irradiation 20 s, then the mutagenic strain Bb10 which had best degradation ability was obtained, and the rate increased from 70.5 % to 88 % . Finally, nitrite re-ductase was separated from strain Bb10, and crude extract with 56.84 U/mL enzyme activity was obtained, 5.33 times higher than pre-mutagenic activity 10.56 U/mL.关键词
乳酸菌/亚硝酸盐/诱变/亚硝酸盐还原酶Key words
lactobacillus/ nitrite/ mutation/ nitrite reductase分类
轻工纺织引用本文复制引用
闫金星,许润博,陈萍,李倩倩,张雪..产亚硝酸盐还原酶的乳酸菌的筛选及诱变[J].吉林农业大学学报,2013,35(1):94-97,4.基金项目
吉林省科技支撑重点项目(20120249) （20120249）
