解剖学杂志2012，Vol.35Issue(6)：705-707,740,4.DOI:10.3969/j.issn.1001-1633.2012.06.001
高血压大鼠第四脑室外侧隐窝室管膜细胞的形态学变化
Morphological observation on ependymal cells in lateral recess of the fourth ventricle in hypertensive rat
摘要
Abstract
Objective:To observe the morphological changes of ependymal cells in the lateral recess of the fourth ventricle (LR4V) of the NO deficient hypertensive rats at different time, as well as the secretion of ependymal cells, and to study the Nestin expression in ependymal cells. Methods: 30 healthy male Wistar rats were randomly divided into three groups: A control group injected with normal saline; Medication group: medication 2-week group; Medication 4-weeks group. Preparation for the animal model of NO deficient hypertensive rats were treated with nitric oxide synthase inhibitor-L-nitro-L-arginine (L-NNA) with 15 mg/d by intraperitoneal injection, and were successfully prepared animal models of hypertensioa Three rats were randomly selected in each group for conventional cardiac perfusion and fixation were done, then the rat brain was taken out and observed under a transmission electron microscope (TEM), and the paraffin sections were stained with nestin immunohistochemical staining. Results: With an increase in blood pressure, the staining of nestin-positive ependymal cells of LR4V showed a trend from deep to shallow. The results of TEM observation: in the control group, the ependymal cells were arranged regularly, with rich cilia and microvilli, and the borders were clear. In the 2-week medication group, the ependymal cells were various in size and shape, and the border was unclear. The nuclei were shrinkage with coarse chromatia There were less cilia on free surface of ependymal cells, "mud-like" discharge can be seen at the top of cells. In the 4-week group, secretory activities of ependymal cells were more vigorous. Conclusion: Under TEM, it is observed that the process of discharge special "mud-like" secretion in ependymal cells of LR4V, and with increasing blood pressure, secretory activities of ependymal cells are more vigorous, indicating that the ependymal cells are sensitive to hypertensioa关键词
高血压模型/外侧隐窝/第四脑室/室管膜细胞/淤泥样分泌物/巢蛋白/透射电镜/大鼠Key words
hypertension model/ lateral recess/ fourth ventricle/ ependymal cells/ sludge-like secretion/ nestin/ transmission electron microscope/ rat引用本文复制引用
杨靖,郭建美,王玉霞,曹翠丽,马常升..高血压大鼠第四脑室外侧隐窝室管膜细胞的形态学变化[J].解剖学杂志,2012,35(6):705-707,740,4.基金项目
河北省卫生厅课题资助项目(2011547) （2011547）
