解剖学杂志2012，Vol.35Issue(6)：800-803,816,5.DOI:10.3969/j.issn.1001-1633.2012.06.025
复方丹参注射液体外诱导人羊膜上皮细胞分化为神经元样细胞及Notch1表达的变化及意义
Expression and effect of Notch1 during differentiation of human amniotic epithelial cells into neuron-like cells induced by salvia miltiorrhiza bunge in vitro
摘要
Abstract
Objective:To explore the expression and effect of Notchl during differentiation of human amniotic epithelial cells (hAECs) into neurons induced by salvia miltiorrhiza bunge (SMB) in vitro. Methods:hAECs were divided into a SMB pre-treatment group and a control group, and SMB group had 5 subgroups. The morphological changes of hAECs were oberved under an optical microscope and the expressions of NSE, MAP-2 and Notchl were identified with the immunocytochemical method. RT-PCR was further used to detect the expression of multiple genes Oct4, Notchl and NSE. The cell viability was measured with methyl thiazolyl tetrazolium (MTT) methods. Results: Under the convert microscope, it was observed that the shape of hAECs started to change, and there were several axon or dendrite-like processes out from the cell body induced by SMB after 24 h. The NSE and MAP-2 positive straining was the strongest in the group of cells in 1. 5 μl/ml at 48 h, while the Notchl positive straining was less than that in the control group. The cell survival rate of every subgroup was decreased compared with the control group. RT-PCR showed that the expression of Oct4 and Notchl was less than that of the control group, wlile NSE was higher than that of the control group. Conclusion: SMB can induce hAECs into neuron-like cells, and the expression of Notchl decreased in the progress (especially at 1. 5μl/ml-48 h). Its possible mechanism may rely on the inhibition of Notch signal pathway.g关键词
人羊膜上皮细胞/神经细胞/复方丹参注射液/诱导分化Key words
human amniotic epithelial cells/ neuron/ salvia miltiorrhiza bunge/ induced differentiation引用本文复制引用
陈旭东,王晓兰,华新宇..复方丹参注射液体外诱导人羊膜上皮细胞分化为神经元样细胞及Notch1表达的变化及意义[J].解剖学杂志,2012,35(6):800-803,816,5.基金项目
河南省教育厅项目(2011C310011) （2011C310011）
