寄生虫与医学昆虫学报2012，Vol.19Issue(4)：199-203,5.DOI:10.3969/j.issn.1005-0507.2012.04.002
重组弓形虫磷酸甘油酸变位酶2联合IL-2、IFN-γ佐剂鼻内免疫小鼠诱导的小肠黏膜免疫应答
INDUCTION OF INTESTINE MUCOSAL IMMUNE RESPONSES IN MICE BY INTRANASAL IMMUNIZATION WITH RECOMBINANT TOXOPLASMA GONDII PHOSPHOGLYCERATE MUTASE 2 PROTEIN COMBINED WITH DIFFERENT ADJUVANTS
摘要
Abstract
In order to analyze the adjuvant effect of IL-2 and IFN-γ, we observed the immune responses including IgA antibody secreting cells (IgA-ASCs) in small intestine mucosal and slgA level in intestine washes which induced by intranasal immunization with recombinant Toxoplasma gondii phosphoglycerate mutase 2 ( rTgPGAM2) proteins combined with IL-2 or IFN--γ in mice. Forty-eight 6-week-old female BALB/c mice were randomly divided into 6 groups. The 8 mice of each immunity group were immunized intranasal three times with 500 IU IL-2, 1000 U IFN--γ, 30 μg rTgPGAM2, 30 μg rTgPGAM2 + 500 IU IL-2 or 30 μg rTgPGAM2 + 1000 U IFN-γ, soluble in total volume of 20 μL of PBS, respectively. The first and the second immunization were 2 weeks of interval, and there was 1 week of interval between the third immunization and the second one. Mice in control group were treated with 20 μL PBS instead. All mice were sacrificed by cervical dislocation 14 days after the third immunization. The positive rates of IgA-ASCs in mice of duodenum, jejunum, and ileum were detected by immunohistochemistry. The slgA in intestinal washes were analyzed with ELISA assay. The results showed that IgA-ASCs positive rate in the immunity groups were higher than that of the control group, especially in rTgPGAM2, rTgPGAM2 combined with IL-2 or IFN-γ adjuvant groups ( P < 0. 05 or P < 0. 01). There was also a significantly higher level of slgA in small intestinal washes of rTgPGAM2 combined with IL-2 or IFN-γ adjuvant groups compared with the control group ( P < 0. 01). We can conclude that intranasal immunization with rTgPGAM2 , TgPGAM2 + IL-2 and TgPGAM2 + IFN-γ effectively induced intestinal mucosal immune responses in mice, IL-2 and IFN-γ significantly enhanced the immune responses to the antigen. As an adjuvant, IL-2 can perform better effect than IFN-γ.关键词
刚地弓形虫/磷酸甘油酸变位酶2/抗体分泌细胞/鼻内免疫/黏膜佐剂Key words
Toxoplasma gondii/ Phosphoglycerate mutase 2/ Antibody secreting cell/ Intranasal immunization/ Mucosal adjuvant引用本文复制引用
祝建疆,李雅清,王海龙,孟晓丽,杨建一,殷国荣..重组弓形虫磷酸甘油酸变位酶2联合IL-2、IFN-γ佐剂鼻内免疫小鼠诱导的小肠黏膜免疫应答[J].寄生虫与医学昆虫学报,2012,19(4):199-203,5.基金项目
国家自然科学基金项目(No.81071374) （No.81071374）
