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首页|期刊导航|寄生虫与医学昆虫学报|蜱IgG结合蛋白的昆虫细胞表达及结合IgG特性分析

蜱IgG结合蛋白的昆虫细胞表达及结合IgG特性分析

秦顺晴 曹杰 周勇志 张厚双 周金林

寄生虫与医学昆虫学报2012,Vol.19Issue(4):235-240,6.
寄生虫与医学昆虫学报2012,Vol.19Issue(4):235-240,6.DOI:10.3969/j.issn.1005-0507.2012.04.007

蜱IgG结合蛋白的昆虫细胞表达及结合IgG特性分析

EXPRESSION OF IGG BINDING PROTEIN IN INSECT CELLS AND ITS IGG BINDING ACTIVITY

秦顺晴 1曹杰 1周勇志 1张厚双 1周金林1

作者信息

  • 1. 中国农业科学院上海兽医研究所,农业部动物寄生虫学重点实验室,上海,200241
  • 折叠

摘要

Abstract

Tick sucks large amount of host blood during its life cycle. Host IgG in blood can pass through tick midgut into haemolymph, and still retain its activity. To protect ticks from damage of host immune response, Immunoglobulin G binding proteins (IGBPs) in tick body can bind with host IgG, then IgG was excreted out. As key moleculars in ticks, IGBPs were regard as the anti-tick vaccine candidate. Rhipicephalus haemaphysaloides are dominant ticks in South China. RH-IGBP was found in its salivary gland, and identified as vaccine antigen. For more details about its bio-function, we constructed the recombinant Bacmid-IGBP, which was expressed successfully by Baculovirus Expression Vector Systems. Then, We detected the recombinant protein by indirect immunofluorescence ( IF A) and Western-blot, and found that its molecular weight was 23 kDa, agreed with what we predicted. We used the purified protein to bind with mammal animals (Rabbit, Pig, Dog, Goat, Mouse, Bovine) and Human IgG, and Rabbit IgG F (abr) 2 and Fc fragment with ELISA. The results suggested that RH-IGBP can bind with IgG from rabbit and pig obviously, and the main recognition site is IgG F (abr) 2 fragment. The results revealed the function mechanism of IGBP preliminarily.

关键词

免疫球蛋白结合蛋白/昆虫细胞表达/镰形扇头蜱

Key words

Immunoglobulin G binding protein/ Baculovirus expression system/ Rhipicephalus haemaphysaloides

引用本文复制引用

秦顺晴,曹杰,周勇志,张厚双,周金林..蜱IgG结合蛋白的昆虫细胞表达及结合IgG特性分析[J].寄生虫与医学昆虫学报,2012,19(4):235-240,6.

基金项目

国家自然科学基金项目(No.31172095) (No.31172095)

寄生虫与医学昆虫学报

OACSTPCD

1005-0507

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