寄生虫与医学昆虫学报2012，Vol.19Issue(4)：241-247,7.DOI:10.3969/j.issn.1005-0507.2012.04.008
四种璃眼蜱ITS2基因的序列分析
SEQUENCE ANALYSIS OF ITS2 GENE IN 4 SPECIES OF HYALOMMA (ACARI:IXODIDAE)
摘要
Abstract
The total DNA was extracted, amplified and sequenced by PCR from 4 species of Hyalomma ticks, Hy. a. asiaticum, Hy. asiaticum, Hy. detritum and Hy. scupense, collected from Junggar Basin of Xinjiang Uygur Autonomous Region. The ITS2 sequences of 6 species of Ixodes were downloaded from GenBank, and then the fragment length of ITS2, the variant sites, G + C contents and the phylogenetic relationships were analyzed with bioinformatics software. It has been recognized that the fragment length of ITS2 sequence in both Hy. asiaticum asiaticum and Hy. asiaticum were 1 458 bp with 3 variant sites ( A ←→G conversion) , 2 of base deletion/ insertion, and the homology reached to 99% ; while the fragment length of ITS2 sequence in both Hy. detritum and Hy. scupense were 1 561 bp with 12 variant sites, the homology reached also to 99% . The G + C contents of ITS2 sequence in the 12 Ixodes ticks were 61. 2% -62. 7% . Phylogenetic analysis indicated that Hy. a. asiaticum and Hy. asiaticum, and Hy. detritum and Hy. scupense were respectively clustered in different branches of phylogenetic tree, and the both branches were then clustered together with Hy. dromedarri and Hy. a. anatolicum. Ticks of Hyalomma, Dermacetor and Rhipicephalus were clustered together forming a big branch, and those of rtaemap/iysaiis lorms alone another big brancn.关键词
蜱/璃眼蜱/ITS2/序列分析/准噶尔盆地Key words
Tick/ Hyalomma/ ITS2/ Sequence analysis/ Junggar Basin引用本文复制引用
张艳艳,刘继荣,薄新文,王开胜,王光雷..四种璃眼蜱ITS2基因的序列分析[J].寄生虫与医学昆虫学报,2012,19(4):241-247,7.基金项目
国家自然科学基金项目(No.31060339) （No.31060339）
