江苏大学学报（医学版）2012，Vol.22Issue(6)：465-467,471,4.
小鼠脑内星形胶质细胞体外培养方法的优化
Optimization on the cultivation method in vitro of cerebral astrocytus from mice
摘要
Abstract
Objective: To optimize the cultivation method of cerebral astrocytes from mice in vitro, in which develop an experimental basis for the functional study of astrocytus. Methods: Cerebral cortex was obtained from 0 to Id C57BL/6 mice and cell suspension was prepared by digestion with trypsin digestion and mechanical dissociation. After differential attachments, primary cells were cultivated for 24 h, so the fresh culture medium instead of the old was added softly. After then, the cell flask was washed shakely before the old culture medium was replaced with the fresh medium every two or three days. After three passages, astrocytus in the culture medium were stained with anti-glial fibrillary acidic protein (GFAP) antibody using immunocytochemical or immunofluorescence staining. Results: After primary cell culture and subculture , the purified astrocytes had the typical shapes with a purity beyond 95% . Conclusion; A reproducible and simple cultivation method in vitro of astrocytus was established, in which high purity astrocytes were obtained.关键词
星形胶质细胞/小鼠/细胞培养/胶质纤维酸性蛋白Key words
astrocytus/ mice/ cell culture/ glial fibrillary acidic protein分类
医药卫生引用本文复制引用
刘晓梅,赵聃,庞蓉蓉,单锴,李妍,王迎伟..小鼠脑内星形胶质细胞体外培养方法的优化[J].江苏大学学报（医学版）,2012,22(6):465-467,471,4.基金项目
江苏省神经退行性疾病重点实验室开放基金资助项目(SJ11KF07) （SJ11KF07）
