扬州大学学报（农业与生命科学版）2012，Vol.33Issue(4)：1-5,5.
鹅细小病毒单克隆抗体的制备及抗原表位区分析
Preparation of monoclonal antibodies against goose parvovirus and primary analysis of their antigenic epitope domains
摘要
Abstract
By using hybridoma cell fusion technology, we succeeded in developing three positive hybridoma cell lines secreting monoclonal antibodies (mAbs) against goose parvovirus (GPV). These prepared mAbs reacted specifically with the purified GPV particles and the VP3 proteins from prokaryotic expressed vector analysis, and had no cross reactivity between these mAbs and duck parvovirus, duck hepatitis virus and goose-originated Newcastle Disease virus in Western bolt. The four overlapped subfragment DNA of the VP3 gene were cloned and expressed in the pCAGGS eukaryotic plas-mid vector, and their reactivities with mAbs were characterized by the indirect immunofluoresce assay (IFA). The result revealed that the mAb 3E8 reacted with two subfragments harboring 135 - 332 amino acid(aa) or 322 - 535 aa. so it can be deduced that the linear epitope domain recognized by the 3E8 mAb was located between 322aa and 332aa of the VP3 protein.关键词
鹅细小病毒/VP3蛋白/单克隆抗体/抗原表位区Key words
goose parvovirus/ VP3 protein/ monoclonal antidody/ epitope domains分类
农业科技引用本文复制引用
王娟,李艳莉,厚华艳,王建业,陶洁,朱国强..鹅细小病毒单克隆抗体的制备及抗原表位区分析[J].扬州大学学报（农业与生命科学版）,2012,33(4):1-5,5.基金项目
国家自然科学基金资助项目(31172317) （31172317）
公益性行业(农业)科研专项(201003012) （农业）
