扬州大学学报（农业与生命科学版）2012，Vol.33Issue(4)：6-9,4.
鸡腺胃分离的传染性支气管炎病毒基因型与致病性研究
Genotype and pathogenicity of infectious bronchitis viruses isolated from proventriculitis cases
摘要
Abstract
To study whether the infectious bronchitis virus (IBV) is the main causative agent of chicken proventriculitis, two IBV strains isolated from proventriculitis cases previously, CK/CH/HeB/2011/1 and CK/CH/HeB/2011/2, were chosed. Firstly, two IBVs were propagated in SPF embryonated chicken eggs, and proved to be far away from any foreign virus pollution. The S1 genes were cloned by RT-PCR, and the sequencing results showed that S1 gene length was 1 611 and 1 620 bp respectively. Two IBVs were further grouped into different genotypes, Mass and QX, through sequence alignment and phylogenetic analysis. To determine the pathogenicity of these two IBVs, two groups of 10 14-day-old SPF chickens were infected with diluted virus (106 EID50 · mL-1) through intranasal route. All chickens in both groups showed respiratory symptoms. Till 10 d postchallenge, the mortality was 90% for both groups. The consistent pathological change in all chickens was characterized as interstitial nephritis, and no proventriculitis similar to clinical cases could be observed in any challenged chicken. The results suggested that multiple genotypes of IBV could be isolated from proventriculitis cases, and the studied two IBVs could not induce proventriculitis alone.关键词
鸡腺胃炎/传染性支气管炎病毒/基因分型/致病性Key words
chicken proventriculitis/ infectious bronchitis viruse/ genotyping/ pathogenicity分类
农业科技引用本文复制引用
张小荣,程靖华,陈启稳,杨瑞,王小波,刘秀梵,吴艳涛..鸡腺胃分离的传染性支气管炎病毒基因型与致病性研究[J].扬州大学学报（农业与生命科学版）,2012,33(4):6-9,4.基金项目
国家自然科学基金青年基金项目(31101815) （31101815）
国家现代农业产业技术体系项目(CARS-41-K08) （CARS-41-K08）
