扬州大学学报（农业与生命科学版）2012，Vol.33Issue(4)：28-32,5.
猪LBP基因电子克隆及生物信息学分析
Electronic cloning and bioinformatics analysis of the pig LBP gene
摘要
Abstract
The lipopolysaccharide-binding (LBP) gene participates in inflammatory reaction about LPS, belonging to the important immune related gene. Using electronic cloning technology, the LBP gene was cloned by seed sequence based on the human LBP gene (NM004139). The results showed that LBP gene consisted of 1 446 bp, coding 481 ami-no acids. The amino acid sequence had similarity compared with human being (74. 2%), mice (64. 8%) , rat (63. 2%), cattle (77. 1%), dog (74. 6%). Evolutionary tree analysis showed that the pig LBP gene was the nearest to the cattle and furthest from the rat. The bioinformatics analysis showed that the molecular weight of LBP protein was 53. 036 7 ku and the theory isoelectric point was 6. 43. Sub-cellular localization forecast showed that the LBP belonged to secrete proteins in the mitochondria (44. 4%), golgi apparatus (22. 2%). A signal peptide existed in N-terminal, and there might be a schizolysis site in 25 - 26 amino acids. There was a higher hydrophobicity in N-terminal located in the signal peptide. The maximum hydrophobic value was 2. 478 and maximum hydrophilic value was 1. 956. The membrane protein was made up of extracellular domain (1 - 6 amino acids), transmembrane region (7 - 29 amino acids), intracellular domain (30 - 481 amino acids). There were nine Ser, eight Thr, two Tyr, which might be the protein kinase phosphorylation site. The structure of extracellular region of LBP protein showed a forniciform helix structure, and consisted of a lot of α-helix in inside and β-sheet in outside of the arc and they arranged parallely and alternately. There were BPI1 and BPI2 conservative structure domain in 33 - 256 and 271 - 474 amino acid residues.关键词
猪/LBP基因/电子克隆/生物信息学Key words
pig/ LBP gene/ electronic cloning/ bioinformatics分类
农业科技引用本文复制引用
喻礼怀,王靖,傅聪,顾雯雯,王龙..猪LBP基因电子克隆及生物信息学分析[J].扬州大学学报（农业与生命科学版）,2012,33(4):28-32,5.基金项目
江苏省科技支撑计划苏北专项(BN2012029) （BN2012029）
江苏省自然科学基金资助项目(BK2012679) （BK2012679）
扬州市科技支撑计划项目(YZ2011071) （YZ2011071）
