扬州大学学报（农业与生命科学版）2012，Vol.33Issue(4)：33-38,6.
饥饿对克氏原螯虾消化酶和抗氧化酶活性的影响
Effects of starvation on digestive enzyme and antioxidant enzyme activities of Procambarus clarkii
摘要
Abstract
This study was conducted to investigate the effect of starvation on major digestive enzymes (gastrointestinal pepsin, hepatopancreatic lipase and gastrointestinal amylase) , antioxidant enzymes [serum superoxide dismutase (SOD) and hepatopancreatic catalase (CAT)] of the red swamp crayfish (Procambarus clarkii). The results showed that the activities of gastrointestinal pepsin and hepatopancreatic lipase decreased gradually as the starvation time extended. On the lst, 2nd and 4th day of starvation, the activities of gastrointestinal pepsin were 15. 80, 14. 01 and 13. 82 U respectively, which were approximated to that of the control group (P＞0. 05). The variation trend of the hepatopancreatic lipase activity was more obvious, 2 days and 4 days starvation groups were significantly different from control and 1 day starvation group (F＜0. 05). The gastrointestinal pepsin and hepatopancreatic lipase activities in 8 days group were 12.16 and 47. 43 U · g-1 , which showed a significant difference with control (16. 16 and 109. 98 U · g-1) (P＜0. 05). The gastrointestinal amylase activity increased at the beginning of the starvation, and then decreased as the starvation time passed, with its peak on the 4th day of starvation (reached 1. 89 U · mg-1). The starvation also significantly influenced the antioxidant enzyme activities of P. clarkia as the activities of serum SOD and hepatopancreatic CAT began to rise and dramatically decreased after the first day of starvation. The serum SOD activities of P. clarkii starved for 2, 4 and 8 days were 7. 65% , 20. 37% and 28. 66% lowed than control group, and the hepatopancreatic CAT activities were dropped by 36. 73% , 45. 58% and 63. 95% on the 2nd, 4th and 8th day of starvation when compared to control.关键词
克氏原螯虾/饥饿/消化酶/超氧化物歧化酶/过氧化氢酶Key words
Procambarus clarkii/ starvation/ digestive enzyme/ superoxide dismutase/ catalase分类
农业科技引用本文复制引用
芦光宇,刘国兴,陈肖玮,李佳佳,魏万红,唐建清..饥饿对克氏原螯虾消化酶和抗氧化酶活性的影响[J].扬州大学学报（农业与生命科学版）,2012,33(4):33-38,6.基金项目
国家公益性行业(农业)科研专项(201003070) （农业）
