军事医学2013，Vol.37Issue(1)：1-5,5.DOI:10.7644/j.issn.1674-9960.2013.01.001
病原微生物实验室实验操作对室内空气产生微生物污染的研究
Contamination of microbiological aerosol generated by pathogenic microbiological labs
摘要
Abstract
Objective To simulate the microbiological aerosol generated by the pathogenic microbiological lab, and to provide scientific data on risk evaluation and protection measures for lab assistants. Methods Serratia marcescens was used as the; indicator microorganism. Many experimental operations and accidental cases were simulated in the; lab,such as mix-up, accidental fail-off, centrifugal operation, centrifugal tube break,injection infection, anatomization of animals and leakage; of a biolsafety cabinet. The risk of microbiological aerosol was analyzed by quantitative air sampling and natural agar sedimentation methods. Results The concentration of microbiological aerosol was different under laboratory conditions. The highest intensity of the microbiological aerosol of mix-up was 1409 CFU/m3 , accidental fall-off was 9346 CFU/m3 , centrifugal operation was ＜10 CFU/m3 , centrifugal tube break was 138 CFU/m3 , injection infection was more than 8232 CFU/m3 , anatomization of animals was ＜4 and biological safety cabinet leakagc was above 801 CFU/m3 , respectively. Conclusion The; intensity of the; microbiological aerosol generated by pathogenic microbiological labs varies widely. A high concentration of microbiological aerosol can be; generated in a local environment. Experimental skill training and application of primary respiratory proteetion cquipments should be cmphasized.关键词
病原微生物/微生物气溶胶/源强/实验室感染Key words
pathogenic microorganism/microbiological aerosol/intensity of the/source/laboratory infection分类
医药卫生引用本文复制引用
温占波,殷喆,董晓凯,陈咏,杜茜,杨文慧,李劲松,胡凌飞,王洁,李娜,张柯..病原微生物实验室实验操作对室内空气产生微生物污染的研究[J].军事医学,2013,37(1):1-5,5.基金项目
国家科技支撑计划资助项目(2008BAI62B05) （2008BAI62B05）
国家科技重大专项资助项目(2012ZX10004402) （2012ZX10004402）
