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基于mtDNA Cytb序列分析养殖与野生刀鲚群体的遗传多样性

魏广莲 徐钢春 顾若波 李建林 徐跑

江西农业大学学报2012,Vol.34Issue(6):1216-1221,1244,7.
江西农业大学学报2012,Vol.34Issue(6):1216-1221,1244,7.

基于mtDNA Cytb序列分析养殖与野生刀鲚群体的遗传多样性

Studies on the Genetic Diversity of Farmed and Wild Populations of Coilia nasus by Analysing Mitochondrial DNA Cyt b genes

魏广莲 1徐钢春 2顾若波 2李建林 2徐跑2

作者信息

  • 1. 南京农业大学无锡渔业学院,江苏无锡214081
  • 2. 中国水产科学研究院淡水渔业研究中心/农业部淡水鱼类遗传育种和养殖生物学重点开放实验室,江苏无锡214081
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摘要

Abstract

To provide a system analysis of differences in genetic diversity, and scientific evidences of conservation of the germ plasm resources and industrialized artifical culture of Coilia nasus, the genetic diversity and genetic structure of Coilia nasus were assessed from three populations by using mitochondrial Cyt b sequences. The complete mitochondrial Cyt b gene sequence is 1141 bp. Twelve haplotypes were detected from the 22 individuals and the haplotype diversity (h) was 0.922. And 18 variation sites were defined, of which 9 were singleton polymorphic sites and the other 9 were parsimony informative polymorphic sites. The nucleo-tide diversity (Π) was 0.0041, the average nucleotide difference ( K) was 4. 714 and the average genetic distance was 0. 004 2. The result showed that genetic parameters and heredity diversities of farmed Coilia nasus and wild Coilia nasus were higher than those of Coilia nasus taihuensis, which meant fanned Coilia nasus populations still had a high genetic diversity.

关键词

刀鲚/养殖群体/野生群体/细胞色素b/遗传多样性

Key words

Coilia nasus/ farmed population/ wild population/ Cyt b/ genetic diversity

分类

农业科技

引用本文复制引用

魏广莲,徐钢春,顾若波,李建林,徐跑..基于mtDNA Cytb序列分析养殖与野生刀鲚群体的遗传多样性[J].江西农业大学学报,2012,34(6):1216-1221,1244,7.

基金项目

国家科技支撑计划项目(2012BAD26B05)、中央级公益性科研院所科研专项(2011JBFA17)、公益性行业科研专项(201203065)、江苏省水产三新工程重大项目(DZ2012-1)和江苏省科技支撑计划(农业)项目(BE2011411) (2012BAD26B05)

江西农业大学学报

OA北大核心CSCDCSTPCD

1000-2286

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