江西农业大学学报2013，Vol.35Issue(1)：38-41,4.
耐盐性不同的大豆品种幼苗的蛋白质组学比较研究
Comparative Analysis of Proteomics in Soybean Cultivar Seedlings with Different Salt Resistance
摘要
Abstract
To investigate the resistance mechanism of different soybean cultivars to salt tolerance, the whole proteins of soybean seedlings from the salt-tolerant cultivar Lee68 and salt-sensitive cultivar N2899 were compared using proteomic analysis. Proteins were extracted using a trichloroacetic acid/acetone protocol, separated by two-dimensional electrophoresis and stained with coomassie brilliant blue. On the pH 4 - 7 2-DE gels there were about 650 protein spots, in which there were 52 differentially expressed spots in abundance between the 2-DE maps of the Lee68 and N2899 soybean seedlings. Then 6 proteins were identified by MAIDI-TOF-MS. The peptide mass fingerprint was searched against the soybean UniGene database and 4 proteins were successfully identified. They were GST 10, GST 9, seed maturation protein PM36 and hypothetical 26.0 ku protein. A possible role of these proteins was discussed between different salt-tolerant cultivars.关键词
大豆/耐盐性/双向电泳/蛋白质组Key words
soybean/ salt tolerance /2-DE/proteomics分类
农业科技引用本文复制引用
徐晓燕,唐迪..耐盐性不同的大豆品种幼苗的蛋白质组学比较研究[J].江西农业大学学报,2013,35(1):38-41,4.基金项目
国家重点基础研究发展计划(973计划)项目(2010CB125906) （973计划）
