江西农业大学学报2013，Vol.35Issue(1)：68-73,6.
培养条件对仙鹤草愈伤组织诱导、增殖及总黄酮含量的影响
Effects of Culture Conditions on Callus Induction, Proliferation and the Total Flavonoid Content of Agrimonia pilosa
摘要
Abstract
The cotyledons of Agrimonia pilosa Ledeb were used to study the effect of different culture conditions on callus induction, proliferation and the total flavonoid content through the orthogonal design method. The results showed that: under the condition of 1/2MS + 6 - BA 1.0( mg/L) + NAA 0. 5 (mg/L), at pH 6.5, the callus was induced more easily, and grew well. The condition of 1/2MS + 6 - BA 2.0(mg/L) + NAA 1.0(mg/L) , and pH 6.0 was the most proper condition for callus to proliferate. At the same time, the total flavonoid content in callus was the highest under that condition.关键词
仙鹤草/愈伤组织/总黄酮/培养条件Key words
Agrimonia pilosa/ callus/ total flavonoid/ culture conditions分类
农业科技引用本文复制引用
周禄斌,连芳青..培养条件对仙鹤草愈伤组织诱导、增殖及总黄酮含量的影响[J].江西农业大学学报,2013,35(1):68-73,6.基金项目
江西省自然科学基金项目(20114BAB204004) （20114BAB204004）
