江西农业大学学报2013，Vol.35Issue(1)：156-160,5.
定向偶联制备三肽囊素人工抗原及其多克隆抗体的研制
Preparation of Artificial Antigen by Conjugation to the Amino Group on Lysine of Bursin and Production of Polyclonal Antibodies against Bursin
摘要
Abstract
In order to produce polyclonal antibodies against Bursin, and develop immunological detective methods for Bursin (BS) , the amino group on - Lys of Bursin molecules were conjugated to bovine serum albumin ( BSA) or chicken ovalbumin ( OVA) by EDC method. The titer of anti-Bursin antibodies in one BLAB/c mice serum, immunized by BSA-KHG-NH2, was about 1:125000, in which polyclonal antibodies binding to OVA-KHG-NH2 could be inhibited by free Bursin and GKHG-NH2, could not be inhibited by KH-GK peptide. These results indicated that the immunized mice produced specifical antibodies against bursin, and the amino group on-Lys of Bursin were conjugated to BSA or OVA. A competitive indirect ELISA ( CI -ELISA) with the anti - Bursin polyclonal antibodies for detecting Bursin was developed, in which the linear range of the inhibition curve was 15-1 000 ng/mL, and the IC50 was 160 ng/mL.关键词
三肽囊素/人工抗原/多克隆抗体/间接竞争ELISAKey words
Bursin/ artificial antigen/ polyclonal antibodies/ competitive indirect ELISA分类
生物科学引用本文复制引用
邓舜洲,冷闯,张文波,许昌满,胡杨,邹柳,邬向东,谌南辉,朱芝秀..定向偶联制备三肽囊素人工抗原及其多克隆抗体的研制[J].江西农业大学学报,2013,35(1):156-160,5.基金项目
国家自然科学基金项目(31160498)、江西省自然科学基金(2010GZN0046)和江西省教育厅项目(GJJ11402)资助 （31160498）
