江西农业大学学报2013，Vol.35Issue(1)：161-164,4.
肉牛附红细胞体PCR检测方法的建立与初步应用
Establishmen and Application of PCR Method for Detection of Cattle Eperythrozoonosis
摘要
Abstract
In order to establish a diagnostic method of molecular biology that can rapidly and accurately detect cattle Eperythrozoon to survey and prevent and control zoonoses, a pair of specific primers were designed according to the published sequence data 16s ribosomal RNA gene of beef cattle Eperythrozoon from Genbank to amplify genomic DNA of cattle Eperythrozoon. The result showed that the targeted gene fragment was 412 bp in length and 98% identical to the published data. The genomic DNA of Escherichia coli, Toxo-plasm and Actinobacillus pleuropneumoniae was amplified by the method, the result was negative. The sensitivity of the assay was 1.23 ng/μL of DNA. It suggested that the method is a rapid, accurate, specific and sensitive diagnostic method for cattle Eperythrozoon.关键词
肉牛/附红细胞体/PCR/检测方法Key words
cattle/ eperythrozoon/ polymerase chain reaction/detection method分类
农业科技引用本文复制引用
许兰娇,陶清松,黄志海,瞿明仁,何后军,万根..肉牛附红细胞体PCR检测方法的建立与初步应用[J].江西农业大学学报,2013,35(1):161-164,4.基金项目
现代肉牛产业技术体系建设专项资金(nycytx-38)、江西农业大学青年基金(09003305)和江西农业大学硕士启动基金(23032700)资助 （nycytx-38）
