江西农业学报2012,Vol.24Issue(11):53-55,58,4.
小麦全蚀病生防芽孢杆菌的筛选与鉴定
Screening and Identification of Biocontrol Bacillus Strains against Take-all of Wheat
摘要
Abstract
With a disc confrontation assay, 234 bacterial isolates antagonistic to Gaeumannomyces graminis var. tritici, the pathogen of wheat take - all disease, have been isolated from the soils in Yunnan province. Among them a strain Kc controlled 56.65% of the disease in the pot experiment when its suspension of 3.3×107 cfu/mL soaked wheat seeds, showing better than 5 g/L Carbenda-zim. This strain was identified as Bacillus amyloliquefaciens on the basis of its morphological, physiological and biochemical characters , and its 16S rDNA sequence had 99.5% homology with that of Bacillus amyloliquefaciens DSM7.关键词
小麦全蚀病/拮抗细菌/16S rDNA/芽孢杆菌/生物防治Key words
Take - all of wheat/ Antagonistic bacterium/ 16S rDNA/ Bacillus/ Biocontrol分类
农业科技引用本文复制引用
吴贤兵,孙睿揆,吴毅歆,王志远,吴兴兴,毛自朝,何月秋..小麦全蚀病生防芽孢杆菌的筛选与鉴定[J].江西农业学报,2012,24(11):53-55,58,4.基金项目
科技部国际科技合作项目(2009DFA32360). (2009DFA32360)