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小麦全蚀病生防芽孢杆菌的筛选与鉴定

吴贤兵 孙睿揆 吴毅歆 王志远 吴兴兴 毛自朝 何月秋

江西农业学报2012,Vol.24Issue(11):53-55,58,4.
江西农业学报2012,Vol.24Issue(11):53-55,58,4.

小麦全蚀病生防芽孢杆菌的筛选与鉴定

Screening and Identification of Biocontrol Bacillus Strains against Take-all of Wheat

吴贤兵 1孙睿揆 2吴毅歆 1王志远 2吴兴兴 2毛自朝 1何月秋1

作者信息

  • 1. 云南农业大学农学与生物技术学院,云南昆明 650201
  • 2. 云南农业大学农业生物多样性与病虫害控制教育部重点实验室,云南昆明 650201
  • 折叠

摘要

Abstract

With a disc confrontation assay, 234 bacterial isolates antagonistic to Gaeumannomyces graminis var. tritici, the pathogen of wheat take - all disease, have been isolated from the soils in Yunnan province. Among them a strain Kc controlled 56.65% of the disease in the pot experiment when its suspension of 3.3×107 cfu/mL soaked wheat seeds, showing better than 5 g/L Carbenda-zim. This strain was identified as Bacillus amyloliquefaciens on the basis of its morphological, physiological and biochemical characters , and its 16S rDNA sequence had 99.5% homology with that of Bacillus amyloliquefaciens DSM7.

关键词

小麦全蚀病/拮抗细菌/16S rDNA/芽孢杆菌/生物防治

Key words

Take - all of wheat/ Antagonistic bacterium/ 16S rDNA/ Bacillus/ Biocontrol

分类

农业科技

引用本文复制引用

吴贤兵,孙睿揆,吴毅歆,王志远,吴兴兴,毛自朝,何月秋..小麦全蚀病生防芽孢杆菌的筛选与鉴定[J].江西农业学报,2012,24(11):53-55,58,4.

基金项目

科技部国际科技合作项目(2009DFA32360). (2009DFA32360)

江西农业学报

OACSTPCD

1001-8581

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