江西农业学报2012，Vol.24Issue(11)：125-128,4.
猪肺炎支原体168株特异性单抗的制备及鉴定
Preparation and Identification of Monoclonal Antibodies against Mycoplasma hyopneumoniae Strain 168
摘要
Abstract
Balb/c mice were immunized with Mycoplasma hyopneumoniae (Mhp) strain 168, and the splenocytes of the immunized mice were fused with SP2/0 cells. After subcloning for 3 times, four hybridoma clones which produced Monoclonal Antibodies (McAbs) steadily were screened by ELISA, named 2F5, 2G7, 4F5, and 5E9. The result of Western - blot indicated that 2G7 and 4F5 both strongly specifically reacted with the Mhp strain 168. Both of the McAbs (2G7 and 4F5) belonged to IgGl isotype, and their light chains were Κ type. However, 2F5 and 5E9 belonged to IgG2a isotype, and their light chains were also Κ type. They all reacted strongly with Mhp strain 168 in immunofluorescence assay (IFA). The McAbs could be used for the detection and pathogenic mechanism research of Mhp.关键词
猪肺炎支原体/单克隆抗体/间接免疫荧光Key words
Mycoplasma hyopneumoniae/ Monoclonal antibody/ Immunofluorescence assay分类
农业科技引用本文复制引用
王海燕,缪芬芳,冯志新,刘茂军,邵国青..猪肺炎支原体168株特异性单抗的制备及鉴定[J].江西农业学报,2012,24(11):125-128,4.基金项目
江苏省农业自主创新资金资助项目[cx(11)5047]. （11）
