江西农业学报2012，Vol.24Issue(12)：38-41,4.
黑莓‘Kiowa’叶柄离体直接再生体系的建立
Establishment of Direct Regeneration System from in vitro Petioles of Blackberry cv.‘ Kiowa'
摘要
Abstract
Taking the petiole excised from the axenic seedlings of blackberry (Rubus hybrid) cv. ' Kiowa' as explants, a high -frequency in vitro direct regeneration system has been developed. The petioles from 30-40 days' mother cultures on MS medium were used as the initial explants to investigate the influences of medium type, dark treatment time, petiole position, exogenous hormones, and rooting conditions on the plant regeneration. The results showed that: after 30-40 days' subculture of ' Kiowa' test - tube seedlings , culturing the petioles from the top first to the second leaf - position on 1/2MS or MS medium for 14 days or 21 days under dark condition could obtain better regeneration effect. The optimal medium for the inducement of adventitious buds from petioles was MS + CPPU 1.0 mg/L + IAA 0.5 mg/L, and the average regenerated bud number reached 4.42 with the regeneration rate of 87.46%. MS + 6 - BA 0.5 mg/L + IAA 0.1 mg/L was the optimum medium for bud multiplication with the coefficient of 5.19. The optimum rooting medium for petioles was 1/2MS + NAA 0.03 mg/L, in which the rooting rate reached 91.67% and the average number of roots of each plant was 4.00.关键词
黑莓/叶柄/分化/不定芽/再生体系Key words
Blackberry (Rubus hybrid) / Petiole/ Differentiation/ Adventitious bud/ Regeneration system分类
农业科技引用本文复制引用
张春红,王鲁北,王小敏,吴文龙,李维林..黑莓‘Kiowa’叶柄离体直接再生体系的建立[J].江西农业学报,2012,24(12):38-41,4.基金项目
江苏省自然科学基金项目(BK2011386) （BK2011386）
2010年度企业博士集聚计划项目(2010038) （2010038）
江苏省科技基础设施建设计划项目(BM2009041、BM2010458). （BM2009041、BM2010458）
