江西农业学报2012，Vol.24Issue(12)：144-146,3.
纳豆激酶的克隆及在大肠杆菌中的表达
Cloning and Expression of Nattokinase Gene in Escherichia coli
摘要
Abstract
The nattokinase gene pro - NK was cloned by PCR technique. The expression plasmid of Pet32a - pro - NK was constructed based on enzyme digestion and enzyme - linked reaction. The confirmed positive clones were further transformed into Esche-rkhia coli strain BL21(DE3) to study the expression of nattokinase with IPTG induction. The result of SDS -PAGE electrophoretic a-nalysis manifested that nattokinase protein could express efficiently in BL21(DE3).关键词
纳豆激酶基因/克隆/表达/大肠杆菌Key words
Nattokinase gene/ Cloning/ Expression/ Escherichia coli分类
生物科学引用本文复制引用
李敏,陈柳萌,张诚,曾小军,刘芬,陈时建,李文婧,罗武..纳豆激酶的克隆及在大肠杆菌中的表达[J].江西农业学报,2012,24(12):144-146,3.基金项目
江西省科技计划项目"纳豆激酶蛋白在酿酒酵母中的表达及应用"(20112BBF60009). （20112BBF60009）
