昆明医科大学学报2012，Vol.33Issue(12)：26-29,4.
大鼠脑微血管内皮细胞的原代培养
Primary Culture of Rat Brain Microvascular Endothelial Cells
摘要
Abstract
Objective To develop a simple and reproductable method for the isolation and culture of brain microvascular endothelial cells (BMECs) of rats, and provide technical support for the studies of physiology, biochemistry and pharmacology of the brain endothelium and the studies of pathophysiology of cerebrovascular disease. Methods Relatively pure cortex explants were obtained froml-to 4-day-old neonatal Wistar rats by careful dissection, we used cortex explant to culture BMECs and observed the morphology and migration of endothelial cells from cortex explant. The cultured cells were identified according to the morphology and immunocytochemistry of factor VⅢ-associated antigen. Results We found that the cultured cells began to migrate from cortex explant after 24 hours, showed the spindle-shaped morphology and reached the monolayer confluence after 10-14 days. Immunocytochemistry demonstrated more than 95% of the third generation cultured cells were positive for VⅢ factor protein, which indicated the cultured cells were vascular endothelial cells. Conclusion Explant culture is a economic and easy method for primary culture of pure BMECs.关键词
大鼠/脑微血管内皮细胞/原代培养Key words
Rat:Brain microvascular endothelial cell:Primary culture分类
生物科学引用本文复制引用
李华,马明义,马红艳..大鼠脑微血管内皮细胞的原代培养[J].昆明医科大学学报,2012,33(12):26-29,4.基金项目
泸州医学院附属医院人才基金资助项目(11179) （11179）
