林产化学与工业2013，Vol.33Issue(1)：64-68,5.DOI:10.3969/j.issn.0253-2417.2013.01.012
介孔氧化钛与海藻酸钠固定化木聚糖酶的对比研究
Comparison of Immobilized Xylanase Using Mesoporous Titania and Sodium Alginate as Carriers
摘要
Abstract
In order to improve the stability of xylanase in biocatalysi, mesoporous titania (M-TiO2) and sodium alginate were used to immobilize xylanase. The properties of immobilized xylanase were also investigated in this study. The optimum immobilization by mesoporous titiania was obtained as 20. 36 IU/g xylanase was immobilized at 65℃ for 0.5 h under pH 6.0. The maximal immobilized rate is 93. 37 %. In contrast, the xylanase was immobilized when 35 g/L sodium alginate was selected to handle the enzyme at 65 ℃ for 2 h under pH 6.0 environment in the presence of 20 g/L CaCl2. Maximal immobilized rate is 28.77 %. In regard of the free and immobilized xylanase on sodium alginate, immobilized xylanase by mesoporous titania has better thermo-stability and pH stability.关键词
木聚糖酶/固定化/介孔氧化钛/海藻酸钠/稳定性Key words
xylanase/immobilization/mesoporous titania/sodium alginate/stability分类
化学化工引用本文复制引用
单宗星,韦策,李鑫,宋向阳,张杨,张伟炜,徐晨琛..介孔氧化钛与海藻酸钠固定化木聚糖酶的对比研究[J].林产化学与工业,2013,33(1):64-68,5.基金项目
国家林业局林业公益性行业科研专项(200904017) （200904017）
国家自然科学基金资助项目(31000278) （31000278）
国家高技术研究发展计划资助(2012AA022301) （2012AA022301）
