林产化学与工业2013，Vol.33Issue(1)：117-120,4.DOI:10.3969/j.issn.0253-2417.2013.01.022
HPLC法同时测定凤尾草中5种黄酮类成分的含量
Simultaneous Determination of Five Flavonoids in Pteris multifida Poir. By HPLC
摘要
Abstract
An HPLC method was established to the determination of five flavonoids (vicenin-2, lonicerin, rhoifolin, luteolin and apigenin) in Pteris multifida. Separation was carried out on a Diamonsil C18 column (4. 6 mm×250 mm, 5 μm). The mobile phase was acetonitrile-water (containing 0.1% formic acid) with gradient elution (0-10 min, 15:85; 20-30 min, 25:75; 35-40 min, 50:50). The flow rate was 1.0 ml/min at 25℃ , and the detection wavelength was set at 350 nm. The average recoveries for vicenin-2, lonicerin, rhoifolin, luteolin and apigenin were 97. 18% - 105. 20% ( RSD1. 72% - 3. 20% ), 95.02% -104.92% (RSD 1.96% -2.93%), 98.45% -103.70% (RSD 1.66% -2.70%), 96.41% -104.73% (RSD 1.03% -3.46%), 95.82% -99.34% (RSD 1.85% -3.95%), respectively. These compounds showed good linearity(r≥ 0. 999, n = 5) in the detected concentration ranges. There were no significant differences of mass factions of the five flavonoids from different regions. The total mass factions of the five flavonoids were in the range of 0. 5 - 2. 2 mg/g.关键词
凤尾草/黄酮/高效液相色谱法Key words
Pteris multifida Poir. /flavonoids/HPLC分类
化学化工引用本文复制引用
刘建群,任晓静,舒积成,张锐,张维,潘景行..HPLC法同时测定凤尾草中5种黄酮类成分的含量[J].林产化学与工业,2013,33(1):117-120,4.基金项目
国家自然科学基金资助项目(30960495) （30960495）
