临床口腔医学杂志2013，Vol.29Issue(2)：75-77,3.DOI:10.3969/j.issn.1003-1634.2013.02.005
高氟对成釉细胞内氯离子浓度及pH值影响的研究
The effect of fluoride to the intracellular chloride concentration and pH value of ameloblasts.
摘要
Abstract
Objective: To observe the changes of the intracellular chloride concentration and pH value of ameloblasts been treated with high-fluoride. Method: Ameloblast-like LS8 cell lines was treated with 2 mmol / L NaF for 30 min, and the control cells were regular cultured without any treatment. The new chloride fluorescent probe MQAE was used to test the changes of intracellular chloride concentration, and live cell workstation was used to monitor the intracellular chloride concentration and pH value after the cells were treated with fluoride for 30 min. Result: After being treated with 2 mM NaF, the average green fluorescence intensity of experimental group cells was higher than that of the control group; Live cell workstation results showed that blue fluorescence and green fluorescent of control group become weaker, and two kinds fluorescence intensity of experimental group were increased. Conclusion: fluoride may change the intracellular pH value by affecting the intracellular chloride concentration.关键词
氟/成釉细胞/细胞内氯离子浓度/细胞内pH值Key words
fluoride/ ameloblasts/ ilntracellular chloride concentration/ intracellular pH value分类
医药卫生引用本文复制引用
周密,郝英,段小红..高氟对成釉细胞内氯离子浓度及pH值影响的研究[J].临床口腔医学杂志,2013,29(2):75-77,3.基金项目
国家自然科学基金(81070819 （）
31070835) （）
陕西省科技计划项目(:2009k-06) （:2009k-06）
