临床眼科杂志2012，Vol.20Issue(6)：541-545,5.DOI:10.3969/j.issn.1006-8422.2012.06.023
As2O3对兔角膜碱烧伤后HIF-1及iNOS表达的影响
Effection of As2O3 on expression of HIF-1 and iNOS following corneal alkali burns in the rabbit
胡琦 1王建文 1崔美芝 1周文艳 1王珂萌 1徐洋涛 1罗杰 1俞佳伟1
作者信息
- 1. 150001,哈尔滨医科大学附属第一临床医学院眼科医院
- 折叠
摘要
Abstract
Objective To observe the expression of HIF-1 and iNOS and VEGF following alkali burns in the rabbit cornea and find out the effection of AS2O3 on expression of Hi-land iNOS and VEGF. To study the mechanism of HIF-1 and iNOS inhibition of AS2O3 on the cornea neovascularization. Methods To induce the models of alkai burns in Japanese rabits. Rabbits were randomly divided into 4 groups. Different concentration of AS2O3 were used in the experimental group with subconjunctival injection. And the control group was subconjunctival injection with 0. 3ml 0. 9% of normal saline. CNV was observed everyday with slit lamp microscope after alkali burns 24 hours. To detected the expression of HIF-1 and iNOS and VEGF by immunohistochemical techniq. Results The neovascularization area of cornea and the expression of HIF-1 and iNOS and VEGF after alkali burn with AS2O3 were less than those of contrast( P <0. 05 );The difference was significant with the increasing of the dose of AS2O3. Conclusion AS2O3 can inhibit the cornea neovascularization after corneal burns; HIF-1 and iNOS are important angiogenic factors in the process of forming corneal neovascularization; The mechanism of AS2O3 inhibiting corneal neovascularization is probably associated with the inhibitive expression of HIF-1 and iNOS.关键词
兔/角膜/碱烧伤/新生血管/三氧化二坤/缺氧诱导因子-1/诱导性一氧化碳合成酶Key words
AS2O3/ Cornea/ Alkali burn/Neovascularization/ HIF-1/ iNOS引用本文复制引用
胡琦,王建文,崔美芝,周文艳,王珂萌,徐洋涛,罗杰,俞佳伟..As2O3对兔角膜碱烧伤后HIF-1及iNOS表达的影响[J].临床眼科杂志,2012,20(6):541-545,5.
