环境与职业医学2012，Vol.29Issue(11)：684-685,2.
温室土壤有机提取物遗传毒性研究
Genotoxicity of Extractable Organic Matter in Greenhouse Soil
摘要
Abstract
[ Objective ] To study the genotoxicity of extractable organic matter (EOM) in vegetable greenhouse soil. [ Methods ] EOM obtained by soxhlet extraction from greenhouse soil were fed to 3 groups of mice via gavage at concentrations of 5, 15, and 30 g soil dry weight/kg body weight, once per day for 4 weeks. A negative control group was administered with dimethyl sulfoxide and a positive control group with cyelophosphamide. Chromosomal damage in peripheral blood lymphocytes and DNA injury in peripheral blood cells were detected by cytokinesis-block micronucleus (CBMN) assay and comet assay independently. [ Results ] There were significant differences in the micronucleus frequency between the EOM exposure groups and the negative control group, and the micronucleus frequency remarkably increased with EOM concentration. Compared with the negative control group, the tail length, tail DNA percent, and Olive tail moment increased in the middle and the high dose groups. [ Conclusion ] EOM extracted from greenhouse soil could induce chromosomal damage and DNA injury in mice.关键词
温室土壤/有机污染物/遗传毒性/胞质分裂阻滞微核试验/彗星试验Key words
greenhouse soil/ extractable organic matter/ genotoxicity/ cytokinesis-block micronucleus assay/ comet assay .分类
医药卫生引用本文复制引用
刘建文,高祥春,邢杰,任有勋,闫绍妹,邱玉刚,翟庆峰..温室土壤有机提取物遗传毒性研究[J].环境与职业医学,2012,29(11):684-685,2.基金项目
山东省医药卫生科技发展计划项目(编号:2009QZ029) （编号:2009QZ029）
