林业科技开发2013，Vol.27Issue(1)：108-110,3.DOI:10.3969/j.issn.1000-8101.2013.01.030
龙凤竹愈伤组织诱导及再分化的试验
Experiment on callus induction and redifferentiation of Pedilanthus tithymaloides var.nanus
摘要
Abstract
The stems of Pedilanthus tithymaloides var. nanus were taken as explant and induced callus formation. The different culture media, the proportion of different plant hormone were investigated. To add different concentration 6-BA and NAA to the medium composed of 9 different plant regeneration medium by orthogonal design L9(34 )were investigated. The results showed that the suitable media NMB was in favor of the induction, the induction medium added 1 mg/L 6-BA and 0.1 mg/L NAA was beneficial to the callus induction. The suitable regeneration medium was NMB containing 2. 0 mg/L 6-BA, 0. 2 mg/L NAA and 5% sucrose, it could improve obviously the frequency of regenerated shoots.关键词
龙凤竹/组织培养/愈伤组织/再分化Key words
Pedilanthus tithymaloides var. nanus/ tissue culture/ callus/ redifferentiation引用本文复制引用
袁云香..龙凤竹愈伤组织诱导及再分化的试验[J].林业科技开发,2013,27(1):108-110,3.基金项目
陕西省教育厅项目(编号:12JK0832) （编号:12JK0832）
陕西省教育厅项目(编号:09JK434) （编号:09JK434）
国家自然科学基金项目(编号:31000410). （编号:31000410）
