棉花学报2013，Vol.25Issue(1)：17-23,7.
培养基、胚龄和激素配比对棉花胚珠离体培养纤维生长发育的影响
Effect of Medium, Ovule Age, and Hormone Combinations on the Fiber Growth and Development of Cotton Ovule Culture in vitro
摘要
Abstract
In order to establish a suitable system for fiber growth and development during cotton ovule culture in vitro, effects of medium types, hormones and hormone combinations, ovule age etc. were studied, using CCRI 49 as material. As well, based on the established experiment system for ovule culture in vitro, the genotype effect in ovule culture was studied. The results showed that BT basic medium is the best medium for cotton ovule culture in vitro. Liquid culture was better than solid one although both of them could induce the fiber growth and development during the ovule culture. All the ovules sampled before flowering and after flowering can be used as explants in ovule culture, but the best one for fiber growth and development was the ovules sampled at flowering day. Hormone was necessary for fiber growth and development during ovule culture in vitro, all the hormones used in the experiment such as GA3, IAA, NAA, Ethylene, and BR could induce the fiber growth and development, and GA3 was best one. The effects of hormone combinations on fiber growth during the ovule culture in vitro were better than that with single hormone individually, and 5.0 μmol·L-1 IAA+5.0 μmol·L-1 GA3 was the best one among the hormone combinations used in the experiment. The fiber growth and development during the cotton ovule culture in vitro was different significantly among the genotypes used in the experiment, and the best hormone or hormone combination might be different for the different genotypes.关键词
棉花/纤维生长/胚珠培养/激素Key words
cotton/ fiber growth/ ovule culture/ hormone分类
农业科技引用本文复制引用
叶春燕,欧婷,陈进红,何秋伶,祝水金..培养基、胚龄和激素配比对棉花胚珠离体培养纤维生长发育的影响[J].棉花学报,2013,25(1):17-23,7.基金项目
国家973计划项目(2010CB126006) （2010CB126006）
转基因生物新品种培育重大专项(2011ZX08005-005) （2011ZX08005-005）
公益性行业(农业)科研专项经费项目(nyhyzx07-052)和国家自然科学基金(30471108、30671325) （农业）
