南京农业大学学报2013，Vol.36Issue(1)：103-107,5.DOI:10.7685/j.issn.1000-2030.2013.01.018
猪肺炎支原体弱毒株与佐剂配合鼻腔免疫对仔猪呼吸道抗体分泌细胞的影响
Effects of intranasal immunization with attenuated Mycoplasma hyopneumoniae strain in combination with adjuvant on the antibody secreting cells in pig respiratory tract
摘要
Abstract
This experiment was conducted to investigate the area changes of IgA and IgG secreting cells in respiratory tract (mainly nasal mucosa, trachea and lung) of piglets after intranasal immunization with attenuated Mycoplasma hyopneumoniae strain in combination with CpG. 30 newborn piglets were randomly divided into 5 groups,and on being 7 days old,each pig was immunized by intramuscular and intranasal routes. The pigs from intranasal immunization group were immunized again 3 days later. The nasal mucosa,trachea and lung were collected at 42 day-post immunization after the pigs were slaughtered. The antibody secreting cells were showed by immunohistochemieal technology- Results showed that the areas of IgA and IgG secreting cells in nasal mucosa and IgA secreting cells in trachea increased significantly after intranasal immunization with attenuated M. hyopneumoniae strain coupled with adjuvant CpG ( P < 0. 01 ). Also the increased areas were found in the pigs from intranasal immunization with attenuated M. hyopneumoniae strain alone and intramuscular immunization group ( P<0. 01) ,but the effect was much unsatisfied when compared to the attenuated M. hyopneumoniae strain coupled with adjuvant group. No IgA and IgG secreting cells in lung were detected. The results demonstrated that intranasal immunization with attenuated M. hyopneumoniae strain coupled with adjuvant CpG could elicit local immune response in the respiratory tract.关键词
猪肺炎支原体/呼吸道/鼻腔免疫/IgA分泌细胞/IgG分泌细胞Key words
Mycoplasma hyopneumoniae/respiratory tract/intranasal immunization/IgA secreting cell/IgG secreting cell分类
农业科技引用本文复制引用
李云锋,王建美,李鹏成,杨倩..猪肺炎支原体弱毒株与佐剂配合鼻腔免疫对仔猪呼吸道抗体分泌细胞的影响[J].南京农业大学学报,2013,36(1):103-107,5.基金项目
国家公益性行业(农业)科研专项(200803020) （农业）
国家自然科学基金项目(31172302) （31172302）
