南京师大学报（自然科学版）2012，Vol.35Issue(4)：63-72,10.
中华绒螯蟹内源性白斑综合症病毒基因EjsWSSV的克隆与鉴定
Molecular Cloning and Characterization of an Endogenous White Spot Syndrome Virus Gene from Eriocheir Japonica Sinensis
摘要
Abstract
Based on expressed sequence tag (EST)and rapid amplification of cDNA ends (RACE)techniques,a WSSV gene designated as EjsWSSV was cloned and characterized from the Chinese mitten crab Eriocheir japonica sinensis.The full-length cDNA of EjsWSSV is 3 864 bp in size and contains an open reading frame of 3 732 bp which encodes a 1 243 amino acid polypeptide (138.25 kDa)with VWA domain.Sequence alignment,structure comparison and some bioinformatics analyses showed that EjsWSSV shared 100％ similarity with WSSV genome ORF16 sequence.The bioinformatics analyses revealed that EjsWSSV had no putative signal peptide found and indicated that it was probably a non-secretory,alpha spiral and non-transmembrane protein.It was probably located in the cytoplasm.The PCR amplification results indicated that EjsWSSV was probably an endogenous virus gene of E.j.sinensis.关键词
中华绒螯蟹/白斑综合症病毒/cDNA末端快速扩增/cDNA克隆/序列分析Key words
Eriocheir japonica sinensis/ WSSV/ RACE/ cDNA cloning/ sequence analyses分类
生物科学引用本文复制引用
解文利,李鹏,靳芳草,周开亚..中华绒螯蟹内源性白斑综合症病毒基因EjsWSSV的克隆与鉴定[J].南京师大学报（自然科学版）,2012,35(4):63-72,10.基金项目
Jiangsu Province Basic Research Program(Natural Science Foundation Funded Project(BK2010541)). （Natural Science Foundation Funded Project(BK2010541）
