南京医科大学学报（自然科学版）2012，Vol.32Issue(11)：1493-1498,6.
LXA4通过p38MAPK/Nrf2信号通路诱导H9c2心肌细胞HO-1高表达
LXA4 increases the expression of HO-1 by activation of p38MAPK/Nrf2 pathway in H9c2 cardiomyocytes
摘要
Abstract
Objective:To investigate the possible mechanisms of signal transduction pathways of heme oxygenase (HO)-1 expression induced by lipoxin A4 (LXA4) in H9c2 cardiomyocytes. Methods:H9c2 cells were exposed to hypoxia followed by reoxygenation with or without pretreatment of LXA4,nuclear factor-E2 related factor 2(Nrf2) inhibitor (ATRA),p38MAPK inhibitor (SB203580),LXA4 combined with ATRA,and LXA4 combined with SB203580. The mRNA transcription and protein expression of HO-1,Nrf2 and p38MAPK was examined by RT-PCR, Western blot and immunofluorescence staining. Results: Compared with hypoxia/reoxygenation group,the mRNA transcription and protein expression of H0-1 in H9c2 cardiomyocytes pretreated with LXA4 increased significantly(P < 0.05). The expression of HO-1 in H9c2 cardiomyocytes decreased (P < 0.05) after treated with ATRA or SB203580. ATRA blocked the nuclear accumulation of Nrf2 and decreased HO-1 protein expression induced by LXA4 pretreatment (P < 0.05). SB203580 inhibited the phosphorylation level of p38MAPK and decreased HO-1 protein expression induced by LXA4 pretreatment(P < 0.05). Conclusion: LXA4 can induce HO-1 over-expression which has protective effect on H9c2 cardiomyocytes of hypoxia/reoxygenation injury. Its mechanism is related to p38MAPK/Nrf2 signal transduction pathways.关键词
脂氧素/血红素加氧酶/核因子E2相关因子2/p38MAPK/缺氧/复氧损伤Key words
lipoxins/heme oxygenase/nuclear factor-E2 related factor 2/p38MAPK/hypoxia/reoxygenation injury分类
生物科学引用本文复制引用
周昱,吴升华,陈筱青,唐艳荣..LXA4通过p38MAPK/Nrf2信号通路诱导H9c2心肌细胞HO-1高表达[J].南京医科大学学报（自然科学版）,2012,32(11):1493-1498,6.基金项目
国家自然科学基金(30973534,81173052) （30973534,81173052）
