南京医科大学学报（自然科学版）2013，Vol.33Issue(1)：32-36,5.DOI:10.7655/NYDXBNS20130107
具有中和功能的抗单体C-反应蛋白单克隆抗体的制备及其初步应用
Characterization and potential application of monoclonal antibody specific to monomeric C-reactive protein
摘要
Abstract
Objective:To prepare monoclonal antibody(MAb)against monomeric C-reactive protein. Methods:BALB/c mice were immunized with mCRP-BSA and the spleen cells of the mice were fused with myeloma cells SP2/0 at the ratio of 5:1. Monoclonal hybridomas were screened by competitive indirect enzyme-linked-immunoadsorbent assay(ELISA)and limited dilution. Mouse ascites were prepared by intraperitoneal injection of hybridoma cells. MAbs to mCRP was purified by Protein G Affinity chromatography methods. The specificity and activity of ascites antibody were determined by Western Blot and indirect ELISA. The protective effects of this antibody influenced on cardiomyocytes were further studied. Results: Strain of hybridoma cell line could secrete MAb stably. After purification,the obtained monoclonal antibody can decrease mCRP induced TGF-P expression on cardiomyocytes. Conclusion: The obtained strain of hybridoma cell line could secrete MAb stably, and purified MAb had protective effect on cardiomyocytes.关键词
单体C-反应蛋白/单克隆抗体Key words
monomeric C-reactive protein/monoclonal antibody分类
医药卫生引用本文复制引用
岳雅丽,王俊宏,方敏,郭妍,程蕴琳..具有中和功能的抗单体C-反应蛋白单克隆抗体的制备及其初步应用[J].南京医科大学学报（自然科学版）,2013,33(1):32-36,5.基金项目
国家自然科学基金(30900602) （30900602）
江苏省科技厅自然科学基金(BK2011382) （BK2011382）
江苏省"六大人才高峰"(2011WSN-029) （2011WSN-029）
