农业环境科学学报2013，Vol.32Issue(2)：354-362,9.DOI:10.11654/jaes.2013.02.021
松花江底泥真菌的分离培养及分子鉴定
Isolation and Molecular Identification of Fungi from the Sediment of Songhua River, China
摘要
Abstract
The current study aims to select proper media to determine the concentration of sediment fungi in the Songhua River, analyze the differences in environmental factors, identify the representative fungi, and explore their potential environmental applications. Different media [i.e. Potato Dextrose agar(PDA), Czapek agar(CA) and Sabouraud's agar(SDA)] were used to determine the concentration of sediment fungi, and the results were compared using Analysis of Variance and Principal Component Analysis. The internal transcribed spacer (ITS) of isolated fungi was sequenced and a phylogenetic analysis was carried out. There were no significant differences among the fungal concentrations at the same sampling point when cultivated using three kinds of different media. However, there were clear differences in their correlation to environmental factors. The number of fungi cultivated on PDA medium exhibited a significant correlation with organic matter, nitrate nitrogen and total nitrogen, while the fungi cultivated on CA medium showed correlation only with nitrate nitrogen. In contrast, fungi cultivated on SDA medium were significantly associated with the total nitrogen content. The number of fungi cultivated on PDA medium was more closely related with environmental variables than that cultivated on the other two media. Hence, PDA was a more suitable medium in the term of environmental monitoring. 20 representative fungi were isolated and subsequent phylogenetic analysis showed that all of the strains could be identified. The results also demonstrated that the ITS region is a reliable element for fungal identification and ecological analysis.关键词
松花江/底泥/真菌/分离培养/核糖体基因转录间隔区Key words
Songhua River/ sediment/ fungi/ isolation and cultivation/ internal transcribed spacer(lTS)分类
资源环境引用本文复制引用
刘杰,丁艳丽,冯伟,王婧妍,毛冠男,王莹莹..松花江底泥真菌的分离培养及分子鉴定[J].农业环境科学学报,2013,32(2):354-362,9.基金项目
国家水专项项目(2008ZX07526-002-01) （2008ZX07526-002-01）
国家自然基金项目(31000247) （31000247）
