农业生物技术学报2012，Vol.20Issue(12)：1457-1462,6.DOI:10.3969/j.issn.1674-7968.2012.12.013
应用免疫荧光技术研究水稻条纹病毒(RSV)侵染介体灰飞虱卵巢的过程
Investigation of the Spatial-temporal Distribution of Rice stripe virus in the Ovary of Insect Vector Laodelphax striatellus by Immunofluorescence
摘要
Abstract
Rice stripe disease, caused by Rice stripe virus (RSV), has led to severe losses in many rice-cultured countries and regions. RSV is transmitted by the small brown planthopper (SBPHs), Laodelphax striatellus Fallen, in a persistent-propagative, and it also can be transmitted transovarially (vertically) by the small brown planthoppers to their offspring. In order to study the transovarial transmission characteristics of RSV by SBPH vectors, we tested viral infections in internal organs and in ovaries of SBPHs with RT-PCR assay and immunofluorescence for virus antigens. Our results showed that only 5.3% of SBPHs contained virus antigens in the ovaries with immunofluorescence for virus antigens, although the virus was detected by RT-PCR in 28.6% of SBPHs that had access to infected plants. Thus, the ovaries would form the transovarial transmission barrier for RSV, which might determine the efficiency of the transovarial transmission of RSV by SBPH vectors. We further investigated the sequential infection of RSV in the ovaries of SBPH vectors with immunofluorescence for virus antigens. Immunofluorescence studies revealed that RSV initially infected the oviduct, and then progressed to the follicular cells of ovarioles. RSV also could accumulate in the germarium and the nutritive cord of the ovary and in the blastodermal cells of the eggs after oviposition. All these results suggested that RSV might spread into the oocyte together with the nutrition transport from the follicular cells of the ovarioles and germarium to the developing oocytes. Based on the fact that the germarium would develop to oocytes and follicular cells, we deduced that RSV might initially infect germarium, which finally formed viruliferous oocytes, although RSV might also spread into oocytes when accompanied by the nutrition transport. Taken together, our results provide a possible mechanism that RSV can exploit the pathway for the nutrition transport into the oocytes, and finally transport into the blastodermal cells of the eggs.关键词
水稻条纹病毒/免疫荧光/灰飞虱/经卵传播/卵巢Key words
Rice stripe virus (RSV)/Immunofluorescence/Laodelphax striatellus Fallen/Transovarial transmission/Ovary引用本文复制引用
吴维,毛倩卓,陈红燕,陈倩,刘启飞,李凡,魏太云..应用免疫荧光技术研究水稻条纹病毒(RSV)侵染介体灰飞虱卵巢的过程[J].农业生物技术学报,2012,20(12):1457-1462,6.基金项目
教育部新世纪优秀人才支持计划(No.NCET-09-0011)和云南省自然科学基金(No.2003C0042M) （No.NCET-09-0011）
