农业生物技术学报2013，Vol.21Issue(1)：12-18,7.DOI:10.3969/j.issn.1674-7968.2013.01.002
转基因玉米NK603基体标准物质研制
Development of Genetically Modified Maize (Zea mays) Line NK603 Matrix Reference Material
摘要
Abstract
The accuracy, reliability and comparability of genetically modified (GM) detection result can not be ensured due to the lack of GM reference material. In this paper, the selected genetically modified (GM) maize (Zea mays) line NK603 and its recipient were grinded and the particle size was about 100 μm. Three different levels of matrix reference materials were gravimetrically prepared. The homogeneity and stability were checked by quantitative real time PCR. The result showed the matrix reference materials were homogenous and stable under the 4°C during one year's stock. The property values determined by gravimetric method and their expanded uncertainties (k=2) of three matrix GM reference material were (0.50+0.10)%, (1.00±0.35)% and (5.00±0.35)%, respectively. Seven different laboratories were chosen to validate the property values of the GM maize reference materials and the result showed that it w as agreed well with the value determined by the gravimetrical method for each level. The uncertainties of the GM reference materials were comparable with those international GM reference material. Thus, the GM maize reference materials can be applied for the GM maize quantitative detection to ensure the detection result accurate and reliable.关键词
转基因玉米/NK603/基体标准物质/实时荧光定量PCRKey words
Genetically modified maize/NK603/Matrix reference material/Quantitative real time PCR引用本文复制引用
董莲华,隋志伟,沈平,余笑波,王晶..转基因玉米NK603基体标准物质研制[J].农业生物技术学报,2013,21(1):12-18,7.基金项目
国家转基因生物新品种培育科技重大专项(No.2008ZX08012-003)和科技支撑项目(No.2008BAK41B01) （No.2008ZX08012-003）
