农业生物技术学报2013，Vol.21Issue(1)：89-96,8.DOI:10.3969/j.issn.1674-7968.2013.01.011
我国部分地区小麦叶锈菌遗传多样性的SSR分析
Genetic Diversity of Puccinia triticina by SSR in Some Regions of China
摘要
Abstract
Wheat leaf rust, caused by Puccinia triticina, is an important epidemic disease of wheat in China. The objective of this research was to understand the molecular genetic diversity and genetic relationship of P. Triticina populations in China, In the research, all the Chinese P. Triticina samples were collected from Hebei, Henan, Shandong and Sichuan Province in 2009 for analyzing by SSR marker. For the leaf rust populations, the average observed number of alleles (Na) per locus was 1.75, the effective number of alleles (Ne) was 1.40, Shannon's information index (Ⅰ) was 0.35, Nei's gene diversity (H) was 0.23, and percent of polymorphic was 75.29%. The result showed that the populations of P. Triticina were considerably genetic diversified and the genetic diversity level of P. Triticina in Hebei, Henan and Shandong was higher than that in Sichuan. UPGMA cluster analysis showed that 4 populations were clustered into 2 groups at similarity coefficient 0.96, Henan, Shandong and Sichuan clustered in group 2, Hebei clustered in group 1, and the closest genetic relationships were found between Henan and Shandong. Genetic differentiation was investigated by analysis of molecular variance(AMOVA). There were about 8.93% of the total variations among populations and about 91.07% of the total variations within populations. The Nm was 6.10. The result showed that the populations of P. Triticina possessed relatively high levels of genetic diversity. There was high genetic similarity among populations, and the genetic relationships were associated with graphical distribution. The main genetic variation came from the same population. The migration of pathogen among the regions was presented, and this conclusion will help to determine the epidemic regions and dispersal routes of wheat leaf rust.关键词
小麦叶锈菌/遗传多样性/SSR标记Key words
Puccinia triticina/Genetic diversity/SSR marker引用本文复制引用
许敏青,王珅,孟庆芳,刘大群..我国部分地区小麦叶锈菌遗传多样性的SSR分析[J].农业生物技术学报,2013,21(1):89-96,8.基金项目
农业部公益性行业(农业)科研专项(No.200903035-14) （农业）
