农业生物技术学报2013，Vol.21Issue(1)：97-105,9.DOI:10.3969/j.issn.1674-7968.2013.01.012
分离自陕西泾阳番茄的番茄黄化曲叶病毒(TYLCD)的分子特征
Molecular Characterization of Tomato yellow leaf curl virus (TYLCD)Infecting Tomato (Lycopersicon esculentum) in Jingyang County of Shaanxi Province
摘要
Abstract
A total of 73 tomato (Lycopersicon esculentum) samples exhibiting stunting, yellowing and leaf-curling symptoms of tomato yellow leaf curl disease (TYLCD) were collected in Jingyang, Shaanxi Province. Using degenerate primer pair PA and PB of geminiviruses and specific primer pair TYT-F and TYT-R of Tomato yellow leaf curl virus (TYLCV), geminiviruses and TYLCV were detected in 70 samples by PCR. The DNA-B and satellite DNA molecules of geminiviruses were not detectable from all these samples with their universal primers respectively. Using rolling circle amplification PCR (RCA-PCR), cloning and sequencing techniques, the complete nucleotide sequence of isolate SX8 (GenBank Accession No. JN412854) was determined to be 2 781 bp and has highly identity of nucleotide sequences (99.9%) with TYLCV-[SD2] (GenBank Accession No. GU199587)isolated from tomato in Shandong Province. The phylogenetic analysis indicated that TYLCV-[SX8] shared high relativity with those previously reported isolates of TYLCV in China.These results suggest that TYLCD in Shaanxi Province is caused by TYLCV which may originate from DNA-A molecules of TYLCV infecting tomato in Shandong Province.关键词
番茄/陕西泾阳/番茄黄化曲叶病/番茄黄化曲叶病毒/分子特征Key words
Tomato(Lycopersicon esculintum Mill.)/Shaanxi jingyang/Tomato yellow leaf curl disease/Tomato yellow leaf curl virus/Molecular characteristics引用本文复制引用
阮涛,杨会房,杨水英,孙现超,青玲..分离自陕西泾阳番茄的番茄黄化曲叶病毒(TYLCD)的分子特征[J].农业生物技术学报,2013,21(1):97-105,9.基金项目
国家自然科学基金项目(No.30971897) （No.30971897）
