热带作物学报2012，Vol.33Issue(11)：1996-2001,6.DOI:10.3969/j.issn.1000-2561.2012.11.015
调控木薯淀粉合成的干涉载体构建及转化木薯的研究
The Construction of RNA Interference Vectors Regulating Cassava Starch Biosynthesis and the Transformation of Cassava
摘要
Abstract
In order to regulate the starch content and constitute of cassava tuber, the RNAi transformation vectors of ADP-glucose pyrophosphorylase small subunit 1(AGPase SU1), soluble starch synthase 1(SSS1)and granule-bound starch synthase 1(GBSS1)were constructed under the driving of sweet potato tuber specific expression Sporamin A promoter, and regeneration cassava SC205 plants were produced through Agrobacterium-mediated infection of SC205 embryonic callus. The above 3 constructed vectors were confirmed by restriction enzyme digestion and sequencing, respectively. Some regeneration lines were confirmed positive by hygromycin selection and PCR technique. The transgenic lines for the purpose of regulating the starch content and constitute of cassava tuber were produced through this research.关键词
木薯/RNA干涉/腺苷二磷酸葡萄糖焦磷酸化酶/可溶性淀粉合成酶/颗粒性淀粉合成酶Key words
RNA interference/ ADP-glucose pyrophosphorylase/ Soluble starch synthase/ Granule-bound starch synthase分类
生物科学引用本文复制引用
崔丽丽,侯学文,韦祖生,谢宝书,黄强,田益农,黎萍,李慧敏,黄惠芳,彭靖茹..调控木薯淀粉合成的干涉载体构建及转化木薯的研究[J].热带作物学报,2012,33(11):1996-2001,6.基金项目
广西壮族自治区主席基金(No.09203-03). （No.09203-03）
