四川大学学报（自然科学版）2013，Vol.50Issue(1)：193-198,6.DOI:10.3969/j.issn.0490-6756.2013.01.036
野生通江百合高频率再生植株的研究
High efficiency of plant regeneration from wild lily (Lilium sargentiae Wilson)
摘要
Abstract
The leaves, petiole and bulbs of wild lily {Lilium sargentiae Wilson) seedling were used as explants in tissue culture. The results showed that the treatment of 0. 1 % HgCl2 corrosive sublimate for 5 min in after removing the seed wings and soaking in 75% alcohol for 30 s had the best sterilization effect. MS+6-BA 1. 0 mg /L+NAA 1. 0 mg/L+sucrose 30 g/L was the best medium to induce callus. The optimal medium for inducing shoots and proliferation was MS+6-BA 2. 5 mg/L+NAA 0.1 mg/L+ sucrose 30 g/L. Its Ave. multiples of propagation was 9. 38 while proliferation rate reached 100%. MS + IBA1. 00mg/L+ sucrose 30 g/L was suitable for the shoots rooting. 93. 3% plantlets with roots were obtained and the survival rate of transplanting reached 95%.关键词
通江百合/种子/组织培养Key words
Lilium sargentiae Wilson/ seed/ tissue culture分类
农业科技引用本文复制引用
易欣,周颂东,何兴金..野生通江百合高频率再生植株的研究[J].四川大学学报（自然科学版）,2013,50(1):193-198,6.基金项目
国家自然科学基金(31270241,31100161,31070166) （31270241,31100161,31070166）
教育部博士点基金(20090181110064) （20090181110064）
科技部基础性研究专项重点项目(2009FY110100) （2009FY110100）
