四川大学学报（自然科学版）2013，Vol.50Issue(1)：199-204,6.DOI:10.3969/j.issn.0490-6756.2013.01.037
微生物降解金针菇栽培袋料中的棉酚研究
Degradation of gossypol in golden mushroom medium by microorganism fermentation
摘要
Abstract
The single-strain, multi-strain composite solid-state fermentation was used for the gossypol-degrading screening. A beer yeast (Saccharomyces cerevisiae) with high efficiency of gossypol-degrading was screened. By single-factor test to determine the initial fermentation medium mushroom detoxification process conditions, the best conditions for a single factor as follows: inoculums level 15% , moisture content 60%, fermentation temperature 40 °C. Response surface analysis disclosed that the factors' interaction between each other is not significant, one factor's effect to detoxification of gossypol wouldn' t be affected significantly if any other factor changed, and the optimal fermentation conditions of gossypol from golden mushroom medium as follows: inoculums level 14. 85% , moisture content 60. 70% , fermentation temperature 40. 15 °C, the content of gossypol reduced from 287. 563 mg/kg to 33. 685 mg/ kg, and the detoxification efficiency was up to 88. 286%.关键词
金针菇栽培袋料/酿酒酵母/发酵/脱毒Key words
golden mushroom medium/ Saccharomyces cerevisiae/ fermentation/ detoxification分类
生物科学引用本文复制引用
孙英成,蒋娟,汪淼,张杰,冯甦,杨志荣,罗璠..微生物降解金针菇栽培袋料中的棉酚研究[J].四川大学学报（自然科学版）,2013,50(1):199-204,6.基金项目
四川省平台项目(09PT-02) （09PT-02）
