水产学报2013，Vol.37Issue(1)：1-8,8.DOI:10.3724/SP.J.1231.2013.38237
雌核发育二倍体鲫鲤及其他倍性鱼cdc2基因cDNA全序列克隆及表达
The cloning of cdc2 cDNAs and a comparative study of its expression in different ploidy fishes including the diploid gynogenetic hybrid of red crucian carp × common carp
摘要
Abstract
Cdc2(Cyclin Dependent Kinase,namely CDK1)encoded by cdc2 gene and CyclinB combination regulates G2/M transition. To find out molecular mechanism that diploid hybrid fish could produce diploid gamete,the full length cDNAs of cdcl in the third gynogenetic generation(G3) ,red crucian carp( Carassius auratus red var. ) ,triploid crucian carp and allotetraploid were obtained by PCR and rapid amplification of cDNA ends. Our data showed that all the cDNAs of cdcl gene in the four different ploidy fishes encode a protein of 302 amino acids containing a domain (PSTAVRE) which combine Cyclins. A high homology of 97. 6% of the Cdc2 protein can be drawn by comparing the amino acid sequences in these four fishes, which indicates the higher conservative function and evolution of Cdc2 protein in these four fishes. A comparative expression pattern of cdcl in early-stage gonads of G3 and different ploidy fishes was carried out by Realtime PCR using specific primers against the same sequences of coding regions in the four fishes. The results showed that the expression of cdcl in the ovary of G3 was higher than those of red crucian carp and triploid crucian carp, while lower than that of allotetraploid, which, at the molecular level, indicates existence of polyploid oogonia in early-stage gonads of G3. The higher expression of cdcl in G3 suggests that consecutive S-phase replication may occur without intervening mitosis, which might be related to the formation mechanisms for the diploid eggs generated by diploid hybrids.关键词
雌核发育二倍体鲫鲤/cdc2基因/早期卵巢/二倍体卵子/细胞周期Key words
the diploid gynogenetic hybrid of red crucian carp x common carp/ cdcl gene/ early-stage gonads/ the diploid eggs/ cell cycle分类
生物科学引用本文复制引用
陶敏,刘少军,钟欢,周毅,宋灿,张纯,刘筠..雌核发育二倍体鲫鲤及其他倍性鱼cdc2基因cDNA全序列克隆及表达[J].水产学报,2013,37(1):1-8,8.基金项目
国家自然科学基金青年基金项目(31001105) （31001105）
湖南省自然科学基金青年基金项目(10JJ4018) （10JJ4018）
淡水生态与生物技术国家重点实验室开放基金(2011FB15) （2011FB15）
高等学校博士学科点专项科研基金资助课题(优先发展领域)(20114306130001) （优先发展领域）
