水产学报2013，Vol.37Issue(1)：55-62,8.DOI:10.3724/SP.J.1231.2013.38316
拟穴青蟹3-磷酸甘油醛脱氢酶基因的克隆与表达
Cloning and expression analysis of the glyceraldehyde-3-phosphatede hydrogenase in the mud crab (Scylla paramamosain)
摘要
Abstract
Glyceraldehyde-3-phosphatede hydrogenase(GAPDH) is one of the basic enzymes that maintain the life activities. In this paper,gapdh gene was isolated from the mud crab,Scylla paramamosain using RT-PCR and RACE methods. The obtained full-length cDNA of GAPDH was 1 440 bp with an open reading frame of 1 008 bp encoding a putative peptide of 335 amino acids. By alignment, the amino acid sequence of 5. paramamosain GAPDH showed high homology with those of some other animals. It suggested gapdh was highly conservative. Real-time PCR showed that the gapdh gene was expressed in various tissues,and highly expressed in thoracic ganglia, eyestalk ganglia, ovary and epidermis. During the ovarian development, the expression of gapdh mRNA was significantly higher at early-developing stage ( Ⅱ stage) and lowest at ripe stage( V stage). It indicated that GAPDH might play an important role in cell mitosis in ovary of the mud crab.关键词
拟穴青蟹/3-磷酸甘油醛脱氢酶/基因克隆/荧光定量PCR/卵巢发育Key words
Scylla paramamosain/ GAPDH/ gene cloning/ real-time RT-PCR/ ovarian development分类
生物科学引用本文复制引用
付春茹,叶海辉,陈学雷,黄辉洋,李少菁..拟穴青蟹3-磷酸甘油醛脱氢酶基因的克隆与表达[J].水产学报,2013,37(1):55-62,8.基金项目
国家自然科学基金项目(40406030,41076081,31272632) （40406030,41076081,31272632）
