水产学报2013，Vol.37Issue(1)：78-85,8.DOI:10.3724/SP.J.1231.2013.38266
初始密度对微小亚历山大藻生长及产麻痹性贝类毒素的影响
Effects of different inoculation densities on growth and toxin production of Alexandrium minutum
摘要
Abstract
Effects of different inoculation densities on growth and toxin production of Alexandrium minutum were studied. Experiments were conducted under conditions of varying inoculation densities (0. 05 × 104, 0.10 ×104,0. 15 × 104,0. 30 × 104 cells/mL). Higher inoculation densities always resulted in shorter lag phase of A. minutum,and an earlier setting in of the steady growth phase. However, the specific growth rate and maximum cell concentration of A. minutum decreased with increasing inoculation densities. The multiplication models developed correlated well with the observations, as the inoculation density increased, the initial-density dependent parameter a also deceased and the reduction of environmental capacity, instantaneous multiplication included. According to MBA analysis, toxicity of A. minutum is typically highest when cells are growing fast in early exponential phase and lowest as growth slows and eventually stops in plateau phase. The PSP-toxin congeners, GTX1 (2. 14 fmol/cell) , GTX2 (2. 08 fmol/cell) , GTX3 (4. 97 fmol/cell) , GTX4 ( 5. 04 fmol/cell) were analysed by high-performance liquid chromatography with fluorescence diction(HPLC-FLD). Result of this study implied that toxin production of the dinoflagellate A. minutum is better in 0.10 × 104 -0. 15 × 104 cells/mL than other condition of inoculations densities.关键词
微小亚历山大藻/初始密度/生长特性/麻痹性贝类毒素Key words
Alexandrium minutum/ inoculation density/ biological characteristic/ paralytic shellfish poison (PSP)分类
农业科技引用本文复制引用
卞中园,杨锡洪,解万翠,章超桦,李翥,杨亚东..初始密度对微小亚历山大藻生长及产麻痹性贝类毒素的影响[J].水产学报,2013,37(1):78-85,8.基金项目
国家自然科学基金项目(31271938) （31271938）
现代农业产业技术体系(贝类48)建设专项 （贝类48）
