水产学报2013，Vol.37Issue(1)：132-140,9.DOI:10.3724/SP.J.1231.2013.38095
交联方法对草鱼皮胶原蛋白海绵性能的影响
Effect of crosslinking method on biological properties of grass carp skin collagen sponges
摘要
Abstract
In this study, the effect of crosslinking method on biological properties of grass carp skin collagen sponge was discussed. Collagen was extracted from skin of grass carp ( Ctenopharyngodon idellus) and collagen sponge was prepared from this collagen. Then,this collagen sponge was crosslinked with different methods,such as UV, dehydrothermal, EDC/NHS and glutaraldehyde crosslinking processes. At the same time, the biological and mechanical properties of those collagen sponges, including degree of crosslinking, denaturation temperature,tensile strength and enzymatic sensitivity in vitro,were evaluated and compared. Experiment results indicated that the grass carp skin collagen was type I collagen. The degree of crosslinking of different crosslinking methods decreased in the order of glutaraldehyde (72. 0% ) > EDC/ NHS(32. 5% ) > dehydrothermal(29. 9% ) > UV( 15. 6% ). Compared with control collagen sponges,the denaturation temperature (67. 4 ℃), tensile strength ( 125. 6 kPa)and enzymatic sensitivity in vitro of collagen sponges crosslinked by glutaraldehyde were significantly improved ( P < 0. 05 ); EDC/NHS crosslinking could lead to obvious increasing in denaturation enthalpy (6. 86 J/g)and moderate improving on tensile strength (98.6 kPa) and enzymatic sensitivity for collagen sponge ( P < 0. 05). The changes of biological and mechanical properties of collagen sponges after being crosslinked by dehydrothermal and UV crosslinking processes were not notable. The results of FTIR showed that glutaraldehyde crosslinking could improve properties of collagen sponges by forming new covalent bond in triple helix structure of collagen, while EDC/NHS crosslinking do that by forming new hydrogen bonds among collagen molecules. This research shows that glutaraldehyde and EDC/NHS crosslinking could lead to obvious improvement in properties of collagen sponges but the influences on properties of collagen sponges after being crosslinked by dehydrothermal and UV crosslinking processes were limited.关键词
草鱼/鱼皮/胶原蛋白/交联Key words
Ctenopharyngodon idellus/ skin/ collagen/ crosslinking分类
轻工纺织引用本文复制引用
汪海波,梁艳萍,李云雁,王敏,方成,汪海婴..交联方法对草鱼皮胶原蛋白海绵性能的影响[J].水产学报,2013,37(1):132-140,9.基金项目
国家自然科学基金面上项目(21076166) （21076166）
武汉市科技局科技攻关计划项目(200920137006) （200920137006）
武汉市农副资源循环利用与新产品开发工程技术中心资助项目(201120637175):湖北省自然科学基金重点项目(2009CDA117) （201120637175）
湖北省高等学校优秀中青年科技创新团队计划项目(T201208) （T201208）
