水产学报2013，Vol.37Issue(2)：245-255,11.DOI:10.3724/SP.J.1231.2013.38329
饲料糖水平对吉富罗非鱼幼鱼生长和肝代谢功能的影响
Effects of dietary carbohydrate levels on growth performance and liver metabolism functions of juvenile tilapia(Oreochromis niloticus)
摘要
Abstract
This study was to investigate the effect of dietary carbohydrate levels on juvenile tilapia (Oreochromis niloticus)growth performance and liver metabolism functions. Eight groups of 1 200 tilapias, in triplicate, with initial body weight of(0. 36 ± 0. 01) g were fed eight experimental diets( similar protein and lipid contents but carbohydrate levels were 10% ,15% ,20% ,25% ,30% ,35% ,40% ,45% .respectively). After 7 weeks feeding, the results showed that: (1) dietary carbohydrate level had significant effects on weight gain rate,survival rate,feed conversion ratio and hepato-somatic index(HIS)in tilapia juvenile(P < 0.05). The growth rate of the fish fed the diet of 35% carbohydrate level was the highest among the treatments, and the feed conversion ratio of the fish fed the diet of 40% carbohydrate level was the lowest. (2) With increasing of dietary carbohydrate,the muscle glycogen and crude fat content gradually increased. However, the glycogen content rose initially and then declined. (3) The activities of serum alanine aminotransferase, aspartate aminotransferase, phosphofructokinase, and the concentrations of serum total protein and total bile acid were all significantly increased with the elevated levels of carbohydrate (P < 0.05). However,the activities of liver esterase and lipoprotein lipase rose initially and then declined. (4) Liver tissues displayed a few lipid vacuolations in hepatocyte cells at 35% . At the carbohydrate level over 40% ,liver histological sections showed a large number of hepatocyte lipid droplets vacuolar degeneration, nuclear migrations and cytoplasm disappearances. The lipid vacuolation areas in liver histological sections basically tallied with the lipid content of the liver, which were consistent with the severity of fatty liver. In conclusion, according to the observation of growth and prevention of late fatty liver disease, the most appropriate proportion of carbohydrate in the fish diet is around 29. 10% -35. 00% .关键词
吉富罗非鱼/饲料糖水平/生长性能/肝代谢功能Key words
Oreochromis niloticus/ dietary carbohydrate levels/ growth performance/ liver metabolism functions分类
生物科学引用本文复制引用
蒋利和,吴宏玉,黄凯,麻艳群,杨淇龄,余德光,钟灵香..饲料糖水平对吉富罗非鱼幼鱼生长和肝代谢功能的影响[J].水产学报,2013,37(2):245-255,11.基金项目
国家自然科学基金项目(31260640) （31260640）
国家"十二五"科技支撑计划(2012BAD25B04) （2012BAD25B04）
广西"十二五"重大科技专项(1123010) （1123010）
广西科技攻关项目(0992014-2) （0992014-2）
