四川医学2012，Vol.33Issue(10)：1681-1683,3.
马拉色菌属种内基因多态性机制的分析
Analysis of intra-specific genetic diversity of Malassezia
摘要
Abstract
Objective To evaluate the reliability of classification of Malassezia species by RAPD and to investigate the mechanism of intra-species genetic diversity of Malassezia. Methods Seventy-four Malassezia clinical isolates were identified and classified by both RAPD and traditional biochemical techniques. The difference of two classification methods was evaluated. ITS1 region of 7 Malassezia furfur isolates was amplified and further investigated by SSCP. Results In most isolates, the result of RAPD classification is in accordance with that by biochemical technique. However, there are still 9 isolates classified into different species by two methods. Besides, polymorphic RAPD patterns can be observed in some isolates of the same specie, trur by SSCP revealed obvious variation of Single-Strand Conformation. Moreover, such variation was in accordance with RAPD result. Conclusion We suggest that intra-species genetic diversity may interfere with molecular biological classification of Malassezia, and that sequence of ITS1 is the sensitive index for intra-species genetic diversity.关键词
马拉色菌属/基因分型/基因多态性/RAPD/SSCPKey words
Malassezia species/ genetic classification/ genetic diversity/ RAPD/ SSCP分类
医药卫生引用本文复制引用
穰真,崔凡,王有为,李薇..马拉色菌属种内基因多态性机制的分析[J].四川医学,2012,33(10):1681-1683,3.基金项目
四川省卫生厅基金(编号:080328) （编号:080328）
