四川医学2012，Vol.33Issue(10)：1690-1692,3.
铁超负荷诱导胰岛细胞凋亡机制的研究
Thestudy of mechanism on iron overload inducing apoptosis of pancreatic islet cells in the wistar rats
吕雪梅 1高赟 2杨波 2陈涛 2龙洋 2田浩明2
作者信息
- 1. 西藏自治区人民医院内分泌科,西藏拉萨850000
- 2. 四川大学华西医院内分泌代谢病研究室,内分泌科,四川成都610041
- 折叠
摘要
Abstract
Objective To investigate the effect of iron overload on apoptosis of pancreatic islet cells in the Wistar rats. Methods Sixty-five male rats of the Wistar strain were divided randomly into four groups: Group A which received repeated in-traperitoneal injections of ferric nitrilotriacetate (FeNTA) , Group B which received the equivalent dose of disodic nitrilotriacetate, Group C which was injected i. p. Diethylenetriaminepentaacetic acid besides FeNTA and Group D that was untreated controls, respectively. Glucose tolerance tests were performed at the beginning and the 10th week. Serum iron and ferritin were measured after performing the intervention. The protein and mRNA expression of Caspase-3、PPAR-γ and NF-KB in pancreatic islets were assessed by immunohistochemistry and in situ hybridization, respectively. Results At 10 week, both the protein and mRNA expression of Caspase-3 as well as NF-KB were significantly higher in Group A than those of the other groups; the protein and mRNA expression of PPAR-γ in Group A were lower as compared with group B or group D; the apoptotic cells of islets in Group A were more than those in the other groups. Conclusion Iron overload may induce oxidative stress and NF-KB activation, inhibit the expression of PPAR-γ and lead to the increased expression of Caspase-3 in islet cells, which were involved in the apoptosis of islet cells.关键词
铁超负荷/胰岛细胞/凋亡Key words
iron overload/ islet cell/ apoptosis分类
医药卫生引用本文复制引用
吕雪梅,高赟,杨波,陈涛,龙洋,田浩明..铁超负荷诱导胰岛细胞凋亡机制的研究[J].四川医学,2012,33(10):1690-1692,3.
