四川中医2012，Vol.30Issue(12)：42-44,3.
健脾化瘀方对胃癌细胞SGC-7901凋亡和周期的影响
Effects of Jianpi Huayu Recipe on Inducing Cell Apoptosis and Cell Cycle of Human Gastric Carcinoma Cell Line SGC-7901
摘要
Abstract
Objective; Observing the effect of Jianpi Huayu Recipe on Inducing Cell Apoptosis and Cell Cycle of Human Gastric Carcinoma Cell Line SGC-7901. Methods: After SGC-7901 cells were treated with Jianpi Huayu Recipe at different concentrations, cell proliferation was observed by MTT assay. The effect of carcinoma cell apoptosis were detected by Annexin V/PI and the retarding effect of cell cycle were determined by flow cytometry ( FCM). Results; ①Compared with the negative control group, Jianpi Huayu Recipe significantly inhibited the proliferation of SGC-7901 cells. The inhibition effect increased with the dose of Jianpi Huayu Recipe and response time; ②When the cells were treated by 2mg/ml and 4mg/ml of Jianpi Huayu Recipe, cell apoptosis was detected by cytometer; ③As compared with the negative control group, when the cells were treated by Jianpi Huayu Recipe, the percentage of the cells in G2/M phase increased obviously in treatment group, while the percentage of the cells in G0/G1 phase increased slightly, and S phase decreased, which showed the cells were arrested at the G2 phase of the cell cycle. Conclusion-. Jianpi Huayu Recipe can inhibit the proliferation of human gastric carcinoma cell line SGC-7901 and can induce this cell apoptosis in vitro.关键词
健脾化瘀方/人胃癌细胞株SGC-7901/细胞增殖/细胞凋亡/细胞周期Key words
Jianpi Huayu Recipe/ Human gastric carcinoma/ cell line SGC-7901 / Cell proliferation/ Apoptosis/ Cell cycle分类
医药卫生引用本文复制引用
徐佳丽,邹玺,周锦勇,王瑞平..健脾化瘀方对胃癌细胞SGC-7901凋亡和周期的影响[J].四川中医,2012,30(12):42-44,3.基金项目
江苏省中医药管理局领军人才项目(LJ200908),江苏省高校优势学科资助项目. （LJ200908）
